Influencing factors for short-term prognosis of liver failure in pregnancy / 临床肝胆病杂志

Liver failure in pregnancy is one of the most serious complications that greatly threaten the safety of pregnant women and parturients, with a high mortality rate and poor prognosis. The short-term prognosis of liver failure in pregnancy can be predicted based on etiology, clinical type and stage of liver failure, dynamic changes of laboratory markers, liver ultrasound, type and number of complications, selection of obstetric treatment, and whether artificial liver support therapy was performed. It is pointed out that it is necessary for clinicians to comprehensively evaluate the short-term prognosis of liver failure in pregnancy, which helps to guide clinical treatment, optimize medical resource allocation, and improve resource utilization.

Research advances in the etiology of liver failure during pregnancy / 临床肝胆病杂志

Liver failure during pregnancy is a serious liver injury caused by various pathogenic factors during pregnancy and is a critical disease that threatens the life of the mother and the fetus. The causes of liver failure during pregnancy are classified into viral factors, non-viral factors, pregnancy-specific factors, and unknown causes. This article describes the research advances in the incidence rates, deaths, and geographical distribution characteristics of liver failure during pregnancy of different etiologies and compares the clinical features of liver failure during pregnancy of different etiologies. It is pointed out that liver failure during pregnancy of different etiologies has entirely different treatment regimens and prognoses, and the etiological diagnosis has important guiding significance in the treatment of this disease. Further examination should be performed for critically ill pregnant women suspected of this disease to determine the etiology, and appropriate treatment should be given as early as possible.

Role of miR-19a in ulcerative colitis in mice / 南方医科大学学报

<p><b>OBJECTIVE</b>To study the role of miR-19a in ulcerative colitis (UC) in mice.</p><p><b>METHODS</b>The target gene of miR-19a was predicted by bioinformatics analysis. The expression of the target protein in UC colon was detected by immunohistochemistry and Western blotting. The target gene was further identified by enhanced green fluorescent protein (EGFP) report vector system.</p><p><b>RESULTS</b>The target gene of miR-19a was TNF-α as predicted by bioinformatics analysis. TNF-α expression was highly expressed in the colonic tissue of UC mice. MiR-19a could inhibit the report gene activity of TNF-α-3'UTR-WT but no that of TNF-α-3'UTR-Mut.</p><p><b>CONCLUSION</b>The target gene of miR-19a is TNF-α, and the binding site is TNF-α 3'UTR. The possible role of miR-19a in UC pathogenesis involves regulation of TNF-α expression in the colon.</p>

Effect of Baitouweng Decoction on intestinal miR-19a expression in mice with ulcerative colitis / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the therapeutic effect of Baitouweng Decoction on dextran sodium sulphate (DSS)-induced ulcerative colitis in mice and explore its mechanism involving miR-19a.</p><p><b>METHODS</b>Forty female c57 mice were randomly allocated into 4 equal groups, namely the normal control group, model group (treated with 3.5% DSS solution), treatment group (treated with DSS+Baitouweng Decoction), and positive control group (treated with DSS+5-ASA). Ulcerative colitis was induced in the mice by feeding them with 3.5% DSS in drinking water, and the mice in the control group were given water only. The disease activity index (DAI) of the mice in each group was recorded daily. Seven days later, the mice were sacrificed for histological examination of the intestines using HE staining; the expression of miR-19a mRNA in the intestines was detected using RT-qPCR.</p><p><b>RESULTS</b>Compared with the control group, the model group showed significantly increased DAI and histological scores, and administration of Baitouweng Decoction significantly lowered the DAI and histological scores of the DSS-treated mice. The expression of miR-19a was lowered following DSS treatment, and Baitouweng Decoction treatment caused an increased miR-19a expression in DSS-treated mice.</p><p><b>CONCLUSION</b>Baitouweng Decoction has therapeutic effects on DSS-induced ulcerative colitis in mice, and this effect is probably mediated by enhancement of miR-19a expression in the intestines.</p>